Supplement + Food Laboratory Testing FAQ

Methods, turnaround, accreditation, sample submission, and results — answered.

Advanced Laboratories answers the questions quality teams ask most about supplement testing: which methods we run, how fast results come back, what accreditation means, and how to submit a sample. We provide third-party analytical testing for dietary supplement, food, cosmetic, personal care, and cleaning product manufacturers

5-day standard turnaround and 24/7 results access through TrendCheck®.

01

General Laboratory Testing Questions

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What Types of Testing Does Advanced Laboratories Offer?

We run third-party analytical testing for dietary supplements, food products, cosmetic and personal care products, and cleaning products. Our capabilities span vitamins, pesticides, heavy metals, minerals, active ingredient testing, identity testing, microbiology, allergens, and preservative efficacy testing, among others.

Our Capabilities

What Is a Certificate of Analysis (COA)?

A Certificate of Analysis (COA) is an official laboratory document that summarizes the testing performed on a sample and the final results. A COA typically includes:

  • Sample identification
  • Test methods used
  • Specifications or acceptance criteria
  • Final results
  • Approval signatures

Manufacturers use COAs for quality assurance, regulatory documentation, supplier verification, and customer records.

What Does ISO 17025 Accreditation Mean?

ISO/IEC 17025 is the international standard for laboratory competence. Accreditation shows that a lab works with validated methods, calibrated equipment, trained personnel, and documented quality systems to produce reliable, defensible data.

Why Is Third-Party Testing Important?

Third-party testing gives you independent verification of product quality, safety, and label accuracy. It helps you:

  • Support regulatory documentation
  • Verify ingredient identity and potency
  • Detect contaminants
  • Protect brand reputation
  • Build customer trust

What Is Turnaround Time (TAT)?

Turnaround time is how long testing takes from sample receipt to final results. We are known for fast turnaround, with most standard analyses completed in as little as 5 business days.

What’s the Difference Between Qualitative + Quantitative Testing?

Qualitative testing answers “is it present?” Quantitative testing answers “how much is present?” For example, Salmonella testing is typically qualitative (absent or present), while Vitamin C potency testing is quantitative (mg/g or mg/serving).

What Is Matrix Interference?

Matrix interference happens when ingredients in a product affect how a test method performs. Complex products such as botanicals, proteins, oils, or highly pigmented materials can interfere with analytical detection or microbial recovery.

Why Do Laboratories Require Product Specifications?

Specifications define the expected acceptance criteria for a product. They help ensure:

  • Appropriate method selection
  • Proper sample preparation
  • Accurate calibration ranges
  • Correct result interpretation

Potency testing, for example, often needs target concentrations so the sample fits properly within the calibration curve.

What Is an Out-of-Specification (OOS) Result?

An out-of-specification (OOS) result falls outside the established acceptance criteria or product specification. We take OOS results seriously, with most receiving an automatic re-run during the 5 business day turnaround at no extra cost.

Why Do Manufacturers Perform Stability Testing?

Stability testing shows how a product performs over time under different environmental conditions, and it supports shelf-life claims. Stability programs are highly individualized, and our team can walk you through the options.

02

Microbiology + Allergen Testing Questions

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What Is Microbiological Testing?

Microbiological testing evaluates products for microorganisms such as:

  • Total aerobic bacteria
  • Yeast and mold
  • Coliforms
  • E. coli
  • Salmonella
  • Staphylococcus aureus
  • Pseudomonas aeruginosa

These tests help verify product safety before products reach consumers.

Why Is Microbiological Testing Important?

Microbial contamination can affect product safety, shelf life, and regulatory standing. Testing helps you catch contamination risks before products ship.

What Methods Does Advanced Laboratories Use for Microbiology Testing?

We use compendial and validated methods, including:

  • USP methods
  • AOAC methods
  • FDA BAM methods
  • PCPC methods

What Do CFU/g + MPN/g Mean?

CFU/g stands for Colony-Forming Units per gram, and MPN/g stands for Most Probable Number per gram. Both estimate the number of viable microorganisms present in a sample.

Does Advanced Laboratories Re-Test OOS Microbiology Results?

Yes. As part of our quality procedures, microbiological OOS results are automatically investigated and re-run to help rule out lab-related contamination.

What Is Preservative Efficacy Testing (PET)?

Preservative efficacy testing (PET) checks whether a preservative system controls microbial growth over time in products such as cosmetics and personal care items.

Why Should Allergen-Free Facilities Perform Routine Testing?

Even facilities designed to be allergen-free can experience unintended cross-contact through raw materials, equipment, or personnel. Routine testing verifies sanitation and provides documented evidence that supports allergen-free claims.

Can Allergens Be Tested Using Environmental Swabs?

Yes. Environmental swab testing supports cleaning validation and production controls by detecting allergen residues on equipment and manufacturing surfaces before production begins.

What’s the Difference Between Gliadin + Gluten Testing?

Gluten is a group of proteins found in wheat, barley, and rye, while gliadin is one of the primary protein components of gluten that analytical testing targets. ELISA methods commonly quantify gliadin and apply a conversion factor to estimate total gluten, in line with industry approaches to gluten-free verification.

03

Chemistry + Contaminant Testing Questions

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What Are Heavy Metals?

Heavy metals are naturally occurring metallic elements that can contaminate raw materials or finished products. The four most commonly tested are:

  • Arsenic
  • Cadmium
  • Lead
  • Mercury

Why Is Heavy Metals Testing Important?

Heavy metals can enter products through soil, water, or raw material sourcing. Testing helps you address safety standards and regulations such as California Proposition 65.

What Is ICP-MS?

ICP-MS (Inductively Coupled Plasma Mass Spectrometry) is a highly sensitive analytical technique we use for heavy metals testing.

What Is ICP-OES?

ICP-OES (Inductively Coupled Plasma Optical Emission Spectroscopy) is used for mineral and elemental analysis where higher concentration ranges are expected.

What Are Pesticide Residues?

Pesticide residues are trace amounts of agricultural chemicals that can remain on botanical ingredients or plant-derived materials after harvesting and processing.

Why Is Pesticide Testing Important for Botanicals?

Botanical ingredients are higher-risk matrices for pesticide contamination because of their agricultural growing conditions and global sourcing.

Why Does Testing Contaminants Matter?

Contaminants arise from multiple sources, so pesticide analysis is typically tested in conjunction with heavy metals, residual solvents, and microbiological testing. Together, these analyses build a comprehensive contaminant profile that supports risk assessment, supplier qualification, and industry expectations.

What Is HPLC?

HPLC (High-Performance Liquid Chromatography) is an analytical technique used to separate and quantify compounds such as vitamins, caffeine, amino acids, and active ingredients.

Why Do I Need to Provide Specifications for Active Ingredients?

Specifications help us choose the correct dilution so the active concentration lands within the validated measurement range of the method. We can still test without them, though that may mean a longer turnaround and a higher price.

Can One Method Cover Every Form of Vitamin?

No. Vitamins exist in multiple chemical forms that behave differently across product matrices, so a single method cannot measure them all accurately. Reliable vitamin testing uses vitamin-specific, matrix-appropriate methods for defensible results.

Are Vitamins on Your ISO Scope?

Yes. All water-soluble and fat-soluble vitamins are on our ISO scope.

What If I Have a Vitamin Form I Don’t See on Your Price List?

If your vitamin form is not listed, you can request it. We are constantly validating new methods and expanding our capabilities.

04

Identity Testing Questions

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What Is FTIR?

FTIR (Fourier-Transform Infrared Spectroscopy) identifies materials and verifies batch-to-batch consistency by analyzing molecular fingerprint patterns.

What Is HPTLC?

HPTLC (High-Performance Thin-Layer Chromatography) confirms raw botanical identity by comparing a sample’s chemical fingerprint against authenticated reference materials.

What’s the Difference Between HPTLC + FTIR?

HPTLC answers “what plant is this?” by comparing a material’s phytochemical fingerprint to known botanical references. FTIR compares overall material composition and answers “does this batch match previous approved batches?” — useful for batch-to-batch consistency, finished-product comparison, non-botanical raw materials, and supplier screening.

Can HPTLC Confirm Every Botanical Ingredient?

Not always. HPTLC is highly useful for many botanical ingredients, but method suitability depends on the material, the available references, and the complexity of the sample. Our team can help determine whether your ingredient is a good fit.

Do I Need Identification Testing if I Already Have a Supplier COA?

Many manufacturers do both. A supplier COA is useful, and independent identity testing adds a layer of verification that confirms the material you received matches the expected identity before it enters production.

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05

Sample Submission Questions

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How Much Sample Should I Send?

Required sample size depends on the test requested and the product matrix. Our minimum and optimal sample amounts are listed on the price list for easy reference.

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How Should Samples Be Packaged?

Seal samples securely, label them clearly, and package them to prevent leakage, contamination, or damage in transit. Microbiology samples should ship in a sterile container. Environmental swabs and plates should ship next-day priority with cold packs.

Can I Rush Testing?

Rush testing may be available depending on lab capacity and the requested analysis. Contact our team to discuss expedited options.

Do I Need to Submit a Sample Submission Form?

Yes. We can’t process your sample without a completed sample submission form. To ensure timely results, pack the form in the same box as your samples. If you forget, no worries—you can email us a copy.

06

TrendCheck® Questions

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What Is TrendCheck®?

TrendCheck® is our client portal, with 24/7 access to:

  • Testing results
  • Sample status tracking
  • Historical records
  • Certificates of Analysis
  • Data visibility across projects

How Does TrendCheck® Help Me in an Audit?

TrendCheck® keeps all of your testing data in one place, so exporting results, trends, and other records for an audit is fast and easy.

Can Historical Reports Be Retrieved Later?

Yes. Historical testing records and reports stay available through TrendCheck® for future reference and audit support.

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Still Have Questions?

Talk to our team, or submit a sample to get started.